Ipswich Town Women will play at Portman Road during the next men's international break.
Joe Sheehan's side, who currently sit fifth in the third-tier table, will face Chatham Town on Saturday, March 23 in a 3pm kick-off.
All four stands will be open for the Tractor Girls' first-ever game in the stadium.
Their home matches are usually played at Felixstowe & Walton United's ground on Sunday afternoons in front of crowds of 500-1,000.
A statement declares it a 'landmark day for the club', with ticket prices to be announced in due course.
Town chief executive Mark Ashton said: "We are delighted to confirm that our women's team will play a fixture at Portman Road.
"There has been a clear passion and interest in a game being held at the stadium and it is fantastic that we now have one confirmed.
"We consistently see great attendances at Portman Road and we urge all supporters to get behind Joe and the team for this game as well.
"This will be an historic day for the club and we want a strong atmosphere for everyone to enjoy."
Tractor Girls boss Sheehan added: "We're excited to be playing in a league game at Portman Road for the first time. It promises to be a memorable occasion, one that we hope will be a catalyst for more positive change, inspiring and providing further opportunities for young players in the future."
Rachel Harris, the newly-appointed general manager of Ipswich Town Women, said: "This is a significant milestone and an important step forward for our women's team.
"Our supporters have been eagerly awaiting a women's fixture to be held at Portman Road and we are excited to give them the chance to be part of an historic occasion and express their support for us on what will undoubtedly be an unforgettable day."
🏟️ 23/03/2024. pic.twitter.com/S7cnVTKgea
— Ipswich Town (@IpswichTown) February 19, 2024
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